Health promotion teaching plan Term Paper Example | Topics and Well Written Essays - 750 words

Health promotion teaching plan - Term Paper Example These may include; Several jobs demand customary contact with other clients at work. Poor or uncooperative relationships with workmates and supervisors can be an impending source of strain. In addition, stress can occur if a person feels isolated or unjustly treated. Poor job relationships can be a consequence of (Blais & Hayes, 2011): Methods and materials applied in teaching border are capturing essential statistics that aim in noting the magnitude of their impact in the society. Materials, mainly questionnaire, pens and books are relevant in this case. Magnitudes of the stress levels are notable in the eventual cases such as physical, work-related and emotional symptoms. The labeling and consideration of the above noted symptoms validate the noting of the range of the symptoms considerable. Ritualistically, numerous or more than one symptom is notably acknowledgeable before a nurse is considered to have a compassion fatigue. Teaching session takes into account the effectiveness desirable for eventual success measuring of the teaching plan. In the process, the teacher is expected to be realistic since he or she is considered the best judge of his or her activities in the entire process. Noting of the expected is definitely insurmountable in the process. With tasks on how to derive and portray the impacts of the plan critical measures on indicating how the measures cropped up is highly relevant in assuming the eventual results of the model (Blais & Hayes, 2011). With the application of the phone, post test and surveys, extensive data is well achieved in realization of each objective

Theme of discovery in the Murder, The Dead and Clay Essay Example for Free

Theme of discovery in the Murder, The Dead and Clay Essay In the three short stories, you can see how all of the characters are discover their destiny, and become self-aware of their condition. In the Dead this is extremely obvious, as Gabriel (the main character), after misreading several signals that were supposed to warn him about the mood his wife was in, realises that he actually does not understand the person he had married, and that he had been fooling himself both intellectually and emotionally. By trying to escape from that paralysis that affected all of Ireland, as the excess of patriotism symbolised by Miss Ivors would prevent him from taking advantage of what the continent offers, he also prevented himself from learning about what he already had. He does not understand his wife, and he never really manages to escape from Irelands influence anyway, what becomes obvious when seeing his reaction towards Miss Ivors accusation. All of these things allow him to realise that, as he will never free himself, he might as well accept his destiny, by starting his journey towards westward. His paralysis now will be complete, what is symbolised by the snow, which falls all over Ireland. In the Clay, another James Joyce story, Maria (the main character) also discovers their destiny and what that predestined condition entitles. It also involves paralysis and the impossibility of breaking that cycle. This time though it is symbolised by the clay she founds in the saucer that was supposed to tell her what destiny awaited her, and, after a second try, a prayer book. I believe that the clay means that she has nothing to expect from the future, nor travel nor marriage. She has no liberty and no chance to develop. Besides, she is obviously not used to be treated nicely because, although she has a great heart, she also has this witch-like appearance, what drives men away. Her reaction when her friends are teasing her, or when a gentleman is nice to her in the bus, shows how much she really wants to really get married. The clay also symbolises death and the prayer book that religion will probably be involved, maybe a convent, before this end finally arrives. That is why her friends get annoyed at the joke made by the pair of girls, because they also realise this. Her song at the end, which is encircled by a dreamy mood, proves that she is not ignorant of her position either. It is also a vicious, unbreakable circle the one that traps and paralyses the actions of the protagonists of The Murder. It is the one caused by cultural differences and the fact that they live in an enclosed society, what limits their individual freedom. Their existence is confined both intellectually and emotionally, and there is also a limit to how much their perspective develops. From the moment that Jelkas father tells Jim that she will not respect him if he does not hit her, and he ignores this advice, she is bound to betray him. This is because this limit in their personal development does not allow her to realise what a life outside her cultural context has to offer. It defines her, it traps her into a defined pattern, one she cannot escape even by marrying outside it and Jim does not understand this. That is why he feels so frustrated inside his marriage, and seeks for outside company. His own background, so simple, is what blinds him from the way of reaching her heart and become a real couple. Is also that difference he cannot accept, as from the beginning we can notice that he is prejudiced against her family what proves how simple his background is, one that does not accept things to be different, that seeks continuity. There is also an element of island mentality that characterises the three stories. Everything is isolated and therefore you do not have as a wide perspective of things as you could. In The dead is a literal island, Ireland. I believe that in Clay, although it also occurs in Dublin, Joyce is actually means to create an isolated circle of people that she knows and places than she visits. These define more her world, as she knows nothing else. In The Murder the kind of rural and isolated society they live in is also quite isolated from external influence, and it revolves solely on the happenings of the towns and farms. People do not know or care of the world outside that small community. In addition to this, we have the element of learning, which is also a common factor in all of the stories, and it is innate in the term discovery. In The Dead is Gabriel the one who learns that he knows nothing, the one who comes to understand that he does not know the woman he married. Yet, I believe that this understanding and this acceptance of his position, which I mentioned earlier is actually pushing him backwards. He tried to move forward, he could not, but it was too difficult to stay in the middle, and intellectually is suffering a regression. In Clay Maria learns her limitations and learns to live with her dreams while, in the Murder is actually the reader who learns something, as the characters cannot do so as they are trapped in that never ending vicious circle that their upbringings and their condition forced them to enter.

Nature And Benefits Of Collaboration

Nature And Benefits Of Collaboration The exact translation of word collaboration from Latin is together in labour. In dictionary, collaboration is defined as work with another or others on a project. However, Henneman et al (1995) stated that defining collaboration in practice is complex, vague, variable and difficult phenomenon where the term can be used inappropriately. According to Baggs and Schmitt (1988), collaboration in nursing and medicine are nurses and physicians working together cooperatively, solving problems by sharing responsibility and making decisions to carry out patient care and treatment. However, this definition was limited due to its meaning because this does not involve the significant contribution of other health care professionals. Professional Partnership In an environment constantly demanding adaptability, cost-effectiveness, and quality betterment, inter-professional collaboration assures re-examination because maximizing nurse-physician collaboration improves patient care and creates gratifying work roles. From personal experience as a sister in Cardiac Surgery Intensive Care Unit, patients require Level 3 care. Patients are mechanically ventilated and sedated on their admission. Most of the patients get extubated and transfer to ward within 48-72 hours but some stay back due to post- operative complications. Patient needs multidisciplinary team (MDT) approach and care during their recovery including Surgeon, anesthetist, Physicians, respiratory technicians, nurses, physiotherapist, dietician, occupational therapist (OT), Pharmacist, and speech and language therapist. As there are complications involved in a patients treatment and care, collaboration among MDT in the clinical practice area is very essential. Multidisciplinary team actually formed in 1950s and 1960s in order to meet the changes that occurring in the medicine there for able to meet patients social, psychological, rehabilitation and environmental needs (Brown 1982). Atwal and Caldwell (2002) conducted a large research study to evaluate how to improve Inter-professional collaboration through multidisciplinary integrated pathway (ICP). But this study found ICP did not improve MDT collaboration, however, this helped to improve the documentation. Another study conducted by Atwal and Caldwell (2006) found that there have been various argument regarding MDT existing in the surrounding however nurses described MDT as a complete myth or idealistic. According to Baggs and Schmitt (1988) collaboration here includes sharing of planning, decision making, problem solving, setting objectives taking responsibility, working co-operatively, communicating and more over coordinating each other. Nature and Benefits of Collaboration Collaboration has several dimensions. It can happen in both face-to-face appointments and via computerized communications such as voice mail and e-mail. It mainly encompasses swapping of outlooks and concepts that contemplates the viewpoints of all the collaborators. The term collaboration should not be misunderstood. Successful professional collaborative correlations require mutual respect and esteem. They also need trust and persistence. It parallely agrees with patient care quality. Collaboration between physicians and nurses is fruitful when role for patient well-being is divided and allocated. Professionalism is fortified when all members take admiration for successful collaboration which in-turn leads to high-quality patient care. Alas, the contribution of nursing towards the boundary limit is often not naive to spot out. Doctors have usually been sighted as the essential income generators for hospitals. Nevertheless, nurses are also significant makers of earnings. The variance in income and gender lead to consequences between the power balance of nurses and medical doctors. (Fagin,1992). The remainder of this assignment focuses on categories of collaborative strategies, namely self-development, team-development, and communication-development strategies, which can intensify nurse-physician collaboration and associated positive patient and nurse outcomes.(refer) Self-Development Strategies A quota of discrete attributes sways the extent of co-operation between professionals in hospitals. Improving emotional maturity, apprehension of the outlooks of others, and evading sympathy fatigue are self-development behaviors that can increase interdisciplinary collaboration. Team-Development Strategies The one of the recent established organizational concepts is the Team development. Collaboration is vital for team growth and success; and advancing positive execution. Team development includes the following tasks: group formation, respectful arrangememts, dispute control, curtail of negative behaviours, and workplace outline to accelerate collaboration.(refer) Communication-Development Strategies A number of nurses and doctors have been tutored how to contact patients in complicated conditions where bad news has to be conveyed or difficult decisions must be formulated. (Quill and Townsend,1991). The strategies include to communicate effectively in emergencies and via electronically. Physicians and nurses fortified their communication skills in these circumstances. Therapeutic Communities Therapeutic community in UK is portrayed as bijou groups where decisions and options are framed involving the patient based on the views of shared duties, authority and evading reliance on professionals. Formidable leadership is needed to have a secured therapeutic community. In UK, this prototype is instigated within prison service. In USA, therapeutic community is used to depict user-runner cliques with a ranking framework and remuneration. Key issues of collaborative partnership The main issues underlying collaborative partnership between physicians and nurses when the attributes of partnership gives out negative impacts such as problems arising between trust in partners, respect for partners, joint working, teamwork and not trying to eliminate boundaries. The studies show that there are certain negative and supervisoral physician behaviour patterns and the nurses find it difficult to cope up with when they are in a partnership. Research have indicated that if nurse-physician co-operation is successful, it do intensify quality of care, ameliorate correspondence and organization of care, decrease patient morbidity and mortality, heighten patient contentment, and increase job satisfaction and retention. The issues underlying in a nurse-physician relationship were dissimilar and hierarchical in ranking, with physicians with an attitude as superiors and nurses as lower ranking subordinates. Nurses have to make counsels in a way that made their proposals appear t o be initiated by the physicians. Nonetheless, nurses were taught that they are professionals and their bond between doctors is as a colleague, not submissive. Regrettably, the viewpoints of some physicians have been insensitive to change and some still view conveying out their command as the nurses foremost duty. However, the correlation between doctors and nurses in hospitals has never been a balanced one. The main differences in this partnership is contrasting levels of prestige and ranking, and distinct sides of the gender gap. The substantial differences between the two professions were on gratification with inter-departmental co-ordination, and doctors are more applauded for the work done and they take more credit; and nurses have more positive attitude towards patients than the doctors. For instance, a physician was more probably to prioritize on lab results and what measures to undertake, hardly recognizing the significance of the information contributed by nurses verbally. Because nursing and medicine demons trate two different intellectuals with differing practice perspectives, disputes can be reckoned between them. The professional fraternization of doctors stresses cure related activities and that of nursing stresses care related behaviours. The last and the most important issue is lack of communication because it causes the safety of patients to be at a risky level due to lack of censorious data, misconceiving information, vague orders over the telephone, and fail to spot noticing changes in status. These issues have the possibilities which lead to grave damage or unpredicted death of patients. Effective clinical practice must emphasize not only on technological framework problems, but also on the human factor. By tackling these issues, health care consortiums have a chance to enhance their clinical results. Critical Analysis of the collaborative partnership with the importance of individual professions The critical analysis has been done and studied from the personal experience as a sister in Cardiac Intensive Unit. In the unit after the common hand over, sister-in-charge assign the patient and staff will take individual hand-over from the previous staff. Then ward-rounds are carried out by Anesthetic consultant, Surgical and anesthetist registrar along with nurse-in- charge. Then, decisions are made regarding patient management, discharge and transfer outs. Anesthetist gets irritated with registrar and nurses when adequate information was not given. As a unit team leader, main responsibility is to pass correct information to the doctors also involve the patient while discussing their treatment and care if they are awake. Patient recovery and condition are normally discussed during hand-over and bedside nurse opinion has been taken into account while making decision. Patients are not being involved while discussing about their condition. Atwal and Caldwell (2006) commented about three types of team working in clinical practice. The first model excluding patient from all team meeting, the second model, consultant performing bedside round, discussing patient condition and asking how they are feeling? Third one including patient in the team meeting. In 2008, part of Essence of care I have undertaken a patient satisfaction survey in my unit. The main suggestion patients given through this audit was that, they have not been involved whilst discussing about their treatment and recovery. They also commented Doctors and nurses stand at the end of the bed and talk, cannot listen what they are talking about, we are worried. The things are changed now most of the doctors introduce self to the patient, discuss ab out their treatment, listen to them and explain whats happening with them. As a team leader in critical care, the responsibility as a sister is to delegate tasks effectively, prioritizing aspects of care, ensure team members are comfortable with the allocation, encourage team members and listening to them. It is important to know the patients entire clinical condition prior to the shift so that nurses can delegate the patients effectively to team members. At times, effective delegation is not possible due to staff shortage and skill mix. In such situation, sister-in-charge works along with them, as junior staffs are always hesitant to speak up because of fear, retaliation or lack of confidentiality. Disagreements are common in decision making regarding patient management and treatment by surgical team and anesthetist in the unit. However, final decision has been taken by Consultant anesthetist since they have more power in the unit. Inter-professional working clearly recommends making considerable changes to this kind of practice by the power and status. Conflicts do occur sometimes between physiotherapist and nurses regarding time arrangement for mobilizing long term sick patient. Physiotherapists are coming to mobilize the patient but the staff may not be ready for that time due to their various role and responsibilities. When the staff disagrees with time they suggest them to do their own they may not be able to help later. This is due to the lack of understanding about each others role. Pritchard (1981) notes that, lack of awareness of roles leads to develop stereotypical attitudes within a MDT. One study conducted by Dally and Sim (2001) found that the physiotherapist doesn t understand the external pressure that nurses facing and the lack of awareness of their professional autonomy and decision making in rehabilitation. Nurses reported that, they often try to minimize conflicts but not always able to resolve disagreement in their satisfaction level as conflict is the one of the main barrier for collaboration (Allen 1997 and Thomas et al 2003). Lack of understanding on each others role and responsibilities are one of the main barriers in collaborative practice between the nurse and other medical practitioners. In order to have mutual respect and value to other professionals need achieving professional competence in your practice area (Bradford 1989 and Stichler 1995). Inter-professional education helps to develop role awareness, effective communication, mutual trust and respect (Barr et al and Freeth 2001). In critical care, teamwork between MDT is very essential saying that Department Of Health in their NHS plan (2000) introduced the importance of implementing individual professional role in the team. There is remarkable evidence showing that, the team without an undefined role is an unsuccessful team. Every individual should be confident in their own professional role. They should also able to carry out their responsibility, exchange and receive information using their skills, knowledge and effective communication. DAmour and Oandasan (2005) stated that acquiring professional satisfaction is the most individual professional outcome. One of the main concerns in the health care system is that, not meeting the health needs (WHO 2002) of the older, sick and vulnerable people despite increasing the expenditure as nurses drive towards evidence-based practice, cost effective with increasing responsibility. Nurses are able to provide only what demanded of them than providing care (Litchfield 2002). Nurse should be able to make clinical judgment and decision making according to the situation for that critical thinking and education is important. It is essential to have staff development which helps providing up to date information, evidence-based practice research knowledge etc. Since NHS is under the cost cutting they are unable to provide enough funding for their own professionals for the development. Each member of the team contributes their knowledge, skills and experience to improve the patient care, so a therapeutic synergy is possible while working with other health care professions. Summary and Conclusion This report illustrates a fundamental model to document an effective collaborative practice. The core model is based on a framework or structure that consists of seven essential elements.: responsibility and accountability, co-ordination, communication, co-operation, autonomy, mutual trust and respect. The model includes a process for identifying the roles and functions of the nurses and physicians. The partnership between nurses and physicians is being studied using theoretical perspectives: team-working, partnership working, patient-professional collaboration, therapeutic communities and power differentials.

Ellen Foster Essay -- essays papers

Ellen Foster The majority of families were once considered perfect. The father went to work everyday, while the mother stayed at home and cared for her two children, â€Å"Henry† and â€Å"Sue†. The children never fought and the parents were involved in all the community events. Our society has grown to accept that there is no such thing as a perfect family. Eleven-year-old Ellen from the book Ellen Foster, by Kaye Gibbons, grows up in a household where her father is an abusive alcoholic and her mother is too sick to complete everyday tasks. By using her positive assets, and learning from her negative assets, Ellen was able to overcome a lot of challenges throughout the book. One would think that six of the most important external assets fall under the category of support. Without support from one’s family and friends, he/she would have to be living in a state of depression. Ellen can claim to have only two of the support assets, which are to be in a caring school environment and to receive support from three or more nonparent adults. In school, her teachers worry about her, and want to know if she is OK: â€Å"The first day back at school my teacher noticed a bruise he put on my arm†¦ she asked me if I had somewhere to spend the night†¦the teacher says everything is OK and she will make the necessary arrangements†. Although Ellen found support from the school psychologist, she also found support from her best and only friend Starletta’s parents. Starletta’s parents told Ellen, â€Å"You come on back when you want to†¦ If he’s there when you get home you come on back here if you want to†¦Ã¢â‚¬  Starletta’s parents are very understanding and they even take Ellen downtown whenever she needs to, buy clothing or food. Even though Ellen found sup... ...family that she grew up in was such a negative environment. It is very possible that she will grow up to be an art teacher. One might think this because she looked up to her art teacher so much and admired her; Ellen’s mind is full of creativity and ideas. When Ellen’s school found out that her dad was abusive to her they put her up at her art teacher’s house. Ellen says â€Å"I came a long way to get here but when you think about it really hard you will see that old Starletta came even farther†¦ And all this time I thought I had the hardest row to hoe† Like Ellen did, it is important for everyone to look back into their life and see what they have learned. Doing so cannot change ones past but only add to their future. Ellen will always carry the horrors of her childhood with her but by using all of her assets that she gained throughout the book her future can be enriched.

Disney Animation And Child Development Film Studies Essay

Disney animated movies captivate the imaginativeness of an copiousness of people with magnetizing music, magnetic characters, and graphic visuals. My grandma, who had lived in poorness through most of her childhood, loved the Disney animated movies as a kid, because they were an flight from world and gave her the hope of a better hereafter. Because Disney life played an of import function in my grandma ‘s childhood, she replicated these memories with her kids and grandchildren through watching Walt Disney animated movies. Although I was excessively immature to retrieve my first experiences with the Disney animated movies, the first experience Disney animated movie that I can pull from memory is the, â€Å" The Little Mermaid † . As a kid, I fell in love with the appealing characters and the enrapturing music from the film. The supporter Ariel was prosecuting to me as a kid because she was funny and yearned to larn about a universe enigmatic to hers. The life of â€Å" The Little Mermaid † is vivacious and shows capturing soundtrack. The secret plan consists of a mermaid who is fascinated with the human universe and finds herself in love with Prince Eric whom she saves from shipwreck. Ariel ‘s male parent, King Triton, is really protective of his girl and is concerned about her wonder for the human universe. In order to be with the one she loves, Ariel turns to the sea enchantress, Ursula, who casts a enchantment upon her to go human. The conditions for Ariel to stay a human are tha t she must acquire a true love ‘s buss from Prince Eric within three yearss, and that if she does non, she will everlastingly be unable to utilize her voice once more. In the terminal, Prince Eric is tricked into falling in love with Ursula and Ariel must to contend off Ursula in order to salvage the land and those she loves. King Triton apologizes for being excessively protective of Ariel and allows her to populate merrily of all time after with Prince Eric. Disney ‘s â€Å" The Little Mermaid † secret plan contains friendly relationships, wonder, effects from heedlessness, continuity, and the dangers of being overprotective. Today, Disney life plays an active function in my life because the films are comparative to loving one ‘s household, encompassing creativeness and wonder, and an inspiration. Disney animated movies hold given me infinite memories that I cherish with both friends and household. I want to go through on the same happy memories I had with my grandma by doing a particular clip to watch Disney ‘s films with my younger household members. Recently, my three-year-old cousin Addy and I watched Disney-Pixar ‘s â€Å" Brave. † Towards stop where the supporter, Merida, hugs both of her parents, Addy asked me if the princess â€Å" loves her ma and dada? † I told her â€Å" yes, of class she does! † She replied â€Å" I love my ma and daddy excessively! † Although my cousin may ne'er retrieve that minute, it ‘s a minute that I will ever retrieve. Walt Disney Animated Studios embraces positive childhood development through their movies. The Disney animated films focus on a assortment of subjects that can animate kids to inquiry, love and imagine. Although these movies may be viewed merely every bit amusement to some people, to others it is a utile tool to educate kids about auxiliary life lessons through an piquant medium. Disney animated movies focal points on animating kids to be speculative, as inquiring and admiration is indispensable in the acquisition procedure. Disney implements the thought of being analytical and funny through characters that are of course speculative with a strong desire to larn. For illustration, Belle from â€Å" Beauty and the Beast, † is a miss with an fact-finding personality and whose avocation is reading. Another character is Alice from â€Å" Alice in Wonderland † who falls down a coney hole and lands in a universe that merely becomes â€Å" funny and curiouser † the longe r she is in Wonderland. Examples of Disney ‘s execution of creativeness are through audio-visual effects in the 1940 movie â€Å" Fantasia † . â€Å" Fantasia ‘s † nucleus thought throughout the movie is to promote one to utilize their imaginativeness to make simple music, forms, and colourss to so go a wondrous complex narrative inspired by orchestrated music. Creativity plays a cardinal function in the Disney film â€Å" Meet the Robinson ‘s † . Lewis, immature discoverer, uses his imaginativeness, creativeness, wonder, and dedication of accomplishing his end of in going a successful discoverer. Another property Disney animated studios incorporates into their plot lines is the importance of loving one another. Teaching immature kids the important and diverse significances of â€Å" love † is indispensable in a kid ‘s development. Many Disney animated movies teach the significance of loving 1s friends, households, and community. Whether it is love for a friend, or love for one ‘s dreams and wants, love dramas an indispensable function in each of Disney ‘s alive films. Examples of different signifiers of love include the 1940 movie, â€Å" Pinocchio † . Pinocchio is marionette who is loved by his Godhead, Geppetto. A faery as if by magic turns Pinocchio into a existent male child and Geppetto loves Pinocchio like his ain boy. In â€Å" The Many Adventures of Winnie the Pooh † , Winnie the Pooh loves all of his friends in the One-Hundred Acres Wood and they all work together with the challenges they face. In â€Å" Pocahontas † , th e Native American princess makes the determination to be with her love John Smith, and attempts to protect her community from contending the English encroachers and to come to peaceable footings. â€Å" The Princess and the Frog † is a narrative about loving and encompassing one ‘s dreams in life. Tiana, the chief character, wishes to carry through her and her late male parent ‘s dream of having a eating house and works difficult to do her dreams come true. Although Disney life promotes healthy kid development and indispensable accomplishments required for acquisition and turning, there are some who believe the movies may play a negative function in a kid ‘s development. Those critics typically believe that Disney films are excessively unrealistic and have secret plans which center on anti-feminism, advancing philistinism and Foster indolence. As Walt Disney had one time said, â€Å" All sketch characters and fabrications must be hyperbole, imitations. It is the really nature of phantasy and fable. † For one to anticipate pragmatism in a kid ‘s narrative is unrealistic within itself. Many of the Disney animated movies are versions of narratives from common people narratives and other signifiers of old literature. The Grimm Brothers foremost written down the common people narratives of â€Å" Snow White † , â€Å" Cinderella † , and â€Å" Sleeping Beauty † , and â€Å" The Little Mermaid † is an version of the narrative by Hans Christian Anderson. Therefore, many of the secret plans behind popular Disney narratives are non created by Disney, but are alternatively Disney animated studio ‘s ain readings of these narratives that are more household orientated than the original narratives themselves. Some women's rightists argue that the Disney princesses set an unrealistic end of how adult females should act and look. They besides believe that it teaches immature misss that a â€Å" Prince Charming † figure exists. However, many Disney princesses do hold character traits outside the traditional, stereotyped â€Å" female † function. In Disney ‘s alive movie, â€Å" Mulan † , Mulan breaks off from the traditional imposts of her civilization of going a stereotyped Chinese married woman that serves her hubby, and alternatively disguises herself as a male soldier to protect her male parent and battle for her state. Mulan does fall in love in t he film with Captain Li Shang, who she originally detests. However, the movie illustrates that Li Shang is non unflawed. Although some soldiers joke around with Li Shang by naming him a â€Å" reasonably boy, † Li Shang proves that he is speculative, a good leader, and logical. Some believe that the Disney princess movies contain unrealistic outlooks of beauty, and insists that the Disney princesses have a detrimental impact on a miss ‘s outlooks and self-pride. However, they are non taking into consideration of the importance of the spectator ‘s age. Many yearlings and immature kids still do non hold the cognitive ability to hold on the construct of an alive character to be â€Å" puting the criterions of beauty † . Then once they are old plenty to understand that society has already placed a criterion for beauty, they are besides old plenty to understand that the princesses are animated characters and are non existent outlooks for life people. Some beside s argue that Disney animated movies promote kids to be mercenary. However, while Disney does sell a battalion of merchandises, it is non the corporation ‘s duty to parent kids. It is alternatively the exclusive duty of a kid ‘s parents to learn against philistinism. Third, the thought that Disney animated movies promotes the unrealistic dream of life ‘s jobs â€Å" as if by magic † disappearance and that working difficult can be avoided is untrue. Many of the Disney animated characters work hard in the narratives. Although Cinderella had a Fairy Godmother assist her in get awaying from her immorality stepmother and step-sisters, one can interpret that into a existent universe scenario that works hard in life, and had a lucky interruption with person being able to assist them accomplish a better life. In the alive movie, â€Å" The Princess and the Frog, † Tiana must works difficult to do a life and saves money to open up a eating house she has ever wanted. Disney animated movies had inspired me during my childhood old ages to show my creativeness and imaginativeness. Disney animated movies promote kids to be funny, inventive and originative, every bit good as encourage kids to love in a assortment of ways to advance healthy childhood development. Many of the Disney animated movies are versions from common people and faery narratives, every bit good as other novels throughout literary history. Although some may non back up kids sing Disney animated movies due to their ain readings of the movies and what they believe the movies portray, it is finally the parent ‘s function to learn their kids of import life lessons and how to construe the films. It is merely the Disney Animation Studio ‘s to entertain both kids and grownups ; their movies do advance a battalion of promoting messages that can interpret a kid to integrate being inventive, funny, and loving into their ain lives. In decision, Disney animated movies promote health y childhood development. Parents and pedagogues should decently integrate these movies for place amusement and for instruction through promoting kids to utilize their creativeness and concentrating on the ethical motives of the narrative. Work Cited â€Å" Walt Disney Quotes. â€Å" A Walt Disney Quotes. Just Disney, Web. 22 Jan. 2013.

Education For Woman Essay

Education is very essential for every one because it is the only education by which we can differentiate between human beings and animals. Education tells us that how can we live in a society that’s why education is important for every one, for both men and women. In past, women did not receive any education at all. They were not allowed to come out of the four walls of their houses. Domestic works were their only education. But now we are living in 21th centaury where there is no any difference between men and women. In this century women have the same respect as men have. They help each other in every sphere. So education should be given to both men and women. But instead of this there are so many people who do not want to educate their women because they think that women do not need education. They think that women are expected to take care of everyone except themselves. They have to take care of the children, stay home, clean up the house, and be the self-denying wife and mother. They think only that the life of a woman is all about getting married, having children, and being bombarded by unimportant details of domesticity. But they do not understand that the education is very important for women not only for them but for a whole family. Because women are the mothers of the future generation. If women are uneducated, the future generations will be uneducated. In day to day life, the real problems are faced first by women and then the same problems are conveyed to men for solution. If the women are educated, they can solve all the problems of their houses. It is said that when ever any men get education it is only useful for him but when ever any women get education it is useful for whole family. An educated woman gives an educated family and an educated family can makes society better. We can not imagine a god society without the education of women. If a woman will not get education how will she manage whole family? Man and woman are like the two sides of a coin. Without one, the other cannot exist. Education women can not only give an educated family but Education of women can also be helpful in eradicating many social evils such as dowry problem, unemployment problem, etc. Social peace can easily be established. A woman has to play three distinct parts in the course of her life in each of which certain duties are expected of her. The first duty of a woman is to  be a good daughter. The second is to be a good wife. And the third is to be a good mother. Education teaches a mother what she should be. It also teaches her how she would do it to be a good daughter, a good wife and a good mother. Only With the help of education women can know their rights .Woman belongs to a weaker section of the society because she suffers from many handicaps due to rigid, outdated social customs and religious practices. But an educated woman cannot be exploited easily. She is aware of her rights and will go any length to defend them. Also, one of a mother’s highest duties is the education of her children at the time when their mind is not amenable to instruction. A child’s whole future life, to a large extent, depends on the teaching it receives in early childhood and it is needless to say that this first foundation of education cannot be well laid by an ignorant mother. Thus education will enable women to make their children, husbands and parents truly happy. Consequently it is very important that women should be educated. On all these grounds female education is a vital necessity.

A clean, well ligted place essays

A clean, well ligted place essays The story A Clean, Well-lighted Place, pivots around difference between a clean and pleasant caf and a dark, not so clean, bar (bodegas) through the conversation between the young waiter and older waiter. The two waiters discuss about an old man who is in the caf and the idea of an existentialist is illustrated through it. An existentialist, insist that the highest good for an individual is to find his or her own unique belief and to find his or her own way without the aid of universal standards. Ernest Hemingway expresses this idea by presenting the old man and older waiter, as lonely men who need a clean, well-lighted place. Although the younger waiter seems to have happiness, Hemingway may indicate otherwise, through dichotomy between the younger waiter and the old man. The old man is depressed and lonely person who needs a place that is clean and pleasant. It is reasonable to suggest that the caf is place where the old man can find comfort. In fact, the old man tried commit suicide, but his niece found him and cut him down because she was fearful of his soul. The Catholics believe that if people, who commit suicide, will go to place called purgatory; thus, it seems that she was only concern for eternal well-being and not concern for his physical and mental well-being. Obviously, the old man cannot go to his family for comfort. Interestingly enough, a week later the two waiters see him in the caf drinking brandy, which may indicate that the comfort he has found is in a clean and pleasant cafe. Arguably, one could oppose that a person who founds comfort in drinking at a cafe will only contribute to more loneliness and depression. So, the old man should go to church to find comfort. This, however, implies that there is only one way for the old man to obtain comfort. From existentialist point of view, an individual can find his or her own comfort through his or her unique bel ...

What Saddam was really thinkin essays

What Saddam was really thinkin essays The chosen supplementary text is an article titled WHAT SADDAM WAS REALLY THINKING written by Johanna McGeary. The source of the article is the Time magazine published on the 18th October 2004. The inner journey in this article is the decision journey that Saddam Hussein undertakes to become who he hoped to be and who he ended up to be. Saddam Husseins journey begins as he had always hoped to dictate how history would view him. In his mind, he was the successor to great Iraqi heroes like Nebuchadnezzar and Saladin... This shows that though the motivation of his idols, he has followed a life path experienced before by another person. (T)he dictators inner motivations and artful deceptions also motivated him through his journey. Saddams psychology powerfully shaped by a deprived and violent boyhood in a village and tribal society, especially by the strong influence of his xenophobic guardian uncle. This shows that at a young age, his life journey was already planned out for him by his uncle. Saddam was awed by science and impressed by the way technology conveyed military power. His mind was tempted to exploit the power of technology to achieve his goal to be a dictator. Saddam faced a critical decision to hide weapons of mass destruction and secretly maintain development programs, which meant that he could face tough sanctions from the U.N. However, Saddam made a calculated decision to destroy the evidence. Saddam has encountered an obstacle in his journey and has to find a new route to his destination. This is evident as he intended to resume developing weapons of mass destruction, however instead, (he) turned his cunning to sanctions busting. Saddam ordered the execution, which was the path he has chosen. However, this path has resulted in ...

Alcoholics and Liver Transplants

Cohen and Martin do not believe that someone that has induced this disease by alcohol abuse is no less deserving of a liver transplant than someone that takes care of their self. They support the moral argument by stating, â€Å"If alcoholics should be penalized because of their moral fault, then all others who are equally at fault in causing their own medical needs should be similarly penalized†. One of the medical arguments that the authors combat is that liver transplants performed on alcoholics have a lower success rate then non-alcoholics. Statistics are presented in the text to argue this notion which really strengthens this aspect of the argument. They also state that a candidate for any other procedure would not be excluded because of the risk of success rate. This argument rises and falls based on what side you’re on. One could assume that most people waiting for a liver, who have never had a drink in their life would be very reluctant to side with this argument. However, an alcoholic or recovering alcoholic that needed a liver would more than likely be behind this passage all the way. One of the weaknesses of this argument was the comparisons that were made with other procedures. A reason why a doctor may be reluctant to transplant a liver to an alcoholic is the scarcity of the organ itself so comparing it to another organ that may be more abundant is asinine. Overall, this argument was moderately effective for the aforementioned reasons but I do not personally agree with the sentiments of the authors.

Rhetorical Analysis Essay Example | Topics and Well Written Essays - 1000 words - 4

Rhetorical Analysis - Essay Example There are always the breathless promos ("Nude man found at mall: Film at 11!"). There are always the two amiable chatting anchors, usually a middle-aged man and a somewhat younger woman. There are the younger roving reporters, featured live at various points around the community or nation, where they chat up the anchors. ("Do you know why the man was wearing no clothes, Jim?" "We're working on that, Susan") There's the joking weatherman, the jock sportscaster, and more recently, the health editor and the lifestyle reporter. In a nation of enormous diversity, there's something both comforting and appalling in knowing that no matter where you are, the local news--like the local McDonalds--is always the same. What makes most of these newscasts pernicious is that they are at the same time so influential and so awful--at least in journalistic terms. In recent years, the local newscast has replaced the network evening news and the newspaper alike as the average American's main source of ne ws: A study by the Pew Research Center for the People and the Press in 1996 found that 65 percent of all adults said they regularly watched the local TV news; only 42 percent reported that they did the same with a network newscast. In about two-thirds of all markets, according to another study, the early-evening local news shows attracted better ratings than the network newscasts that followed them--and the local news is on for a longer time. Though local newscasts have been studied far less systematically than the national news, nearly everyone who has examined their content has come away with the same conclusion. For example: A 1995 study of the local news in 50 major markets by the Rocky Mountain Media Watch found that crime and disaster news make up about 53 percent of the news on local newscasts--the grislier the crime, the better. ("Son shoots mother five times with bow and arrow") Fluff--deemed by the study as 'soft news, anchor chatter, teases, and celebrity items"--takes up about 31 percent of the whole newscast, on average (items such as "Girl reunited with dog" or "How to tango"). An informal 1993 survey by The Washington Post of local newscasts on stations in five big cities found the percentage of stories involving crime, sex, disasters, accidents, or public fears running at anywhere from 46 to 74. In its survey, the Post found local newscast obsessed with murders, serial killers, snakebites, spider bites, tornadoes, mudslides, explosions, and satanic activity. A 1990 study published by the Columbia Journalism Review found that 18 of the 32 stories analyzed on local newscasts were inaccurate or misleading, and the station usually made no attempt to correct the mistakes. A report published the same year in the L.A. Reader, following examination of a week's worth of stories in that market, found stations routinely airing PR footage provided by companies with no acknowledgment that this was what was going on. In a 1991 book examining the local news, Making Local News, Phyllis Kaniss found, among other things, that local TV news reporters are more likely to accept their sources' viewpoints than are print reporters. A Chicago reporter looking at "sweeps week" on Los Angeles television found heavily promoted news stories on lesbian nuns, Geraldo Rivera's love life, and sex after 60. As a critic once put it: The worst scandal the local news could ever uncover is itself. Local news didn't start out this way. Until about 1970, local news--with its

Reformation A Vital Catalyst for Modernity Term Paper

Reformation A Vital Catalyst for Modernity - Term Paper Example As a consequence Calvin, Erasmus, More, Luther (Brinton 205; 234) and other prominent theologians to question the validity of parts of the New Testament as well as other areas of existing religious thought and practice. The reformation, which eventually resulted from this criticism, can therefore to some extent be seen as a distillation of all the important intellectual movements of the age. The papacy meanwhile continued controlling religious activity throughout the European continent and chose to ignore the tide of revolution that swept across its territory, continuing its money-based piety that worked with mechanical precision regardless of human circumstances. According to the historian Crane Brinton (234 pp), there was not one single area of religious observance in the 16th century that was not in one way or other tied to money. The crisis came when Martin Luther put forward his accusations of misconduct of the church – in the vernacular of the people – and the pap acy answered ‘in Latin’. Oblivious to the changes that had taken place, the papacy attempted to combat Luther with the same worn ‘heresy’ charge of centuries ago, except by then the people had found their champions, of which Luther was only one. The grievances these ‘heroes’ made public were those of nearly every ordinary Christian and for the first time, the people were able to understand what was said. There were many complaints, ninety-seven of them, but the most important ones were, that the Church offered ‘salvation’ in exchange for money, that priests were rich and not subject to the law, and that ordinary people had no access to the bible. Thus the impending reformation was not simply an intellectual exercise but it offered... This paper talks about the Reformation - one of the most influential processes throughout European history. from the 13th century onwards, life changed in leaps and bounds in different areas of human society. These ‘stirrings’ could be felt in many parts of the world, but it was in Europe in particular where a concentration of great thinkers provided the fertile ground from which modernity eventually sprang. There were many milestones along the way, in the form of explorations, discoveries, developing insights and wars. But it was foremost the growing disillusionment with the spiritual guidance of the Catholic Church that was of great importance because it affected all people. This ‘spiritual’ uprising swept across Europe and eventually culminated in the ‘Reformation, which questioned and broke papal authority. It opened up opportunities for new ways of thinking and worshipping, made possible the nation state and shaped the modern world through its int ellectual offsprings. The reformation must therefore be seen as the single most important catalyst for modernity. The paper concludes that, at the time the Reformation took place, the groundswell that brought about liberal revolution was strong already but without curtailing of the power of the Catholic Church, progressive thinkers of the 16th and 17th centuries may well have become the victims of heresy trials – as they had in centuries before the reformation. As it was, the ideals of the reformation were allowed to flourish.

Economists Essay Example | Topics and Well Written Essays - 1750 words

Economists - Essay Example Fisher was born in 1876 and received his Business Administration degree and Ph. D in Yale University. As the professor of economics and mathematics at Yale University, Irving Fisher developed extensive concepts concerning money supply and price fluctuations using mathematical evidence (Allen, 1977). Fisher was among the most accurate and leading economists of the 20th century. He used his acquired knowledge and skills from Yale to observe and analyze dramatic and complex macroeconomic events during his era. Fisher also proposed appropriate solutions to the Great Depression that crippled the U.S economy and other aspects of life. The crash of the stock market in the U.S in 1929 and consequential falling of stocks and goods’ prices prompted Fisher to revise his economic theories on price fluctuations, debt and the depression caused by these factors (Mendoza, 2009). Fisher formulated a debt-deflation theory and named the financial crisis, the Great Depression, which is currently used to relate to the economic recession in the U.S.The debt-deflation theory indicates that consumers borrow money for investment on speculation and imagine profits, which leads to losses, debt, deflation and pessimism. Thesis Statement: Irving Fisher has a significance impact on the prevailing U.S economy through his debt-deflation theory, which examines the causes of debt, the deflation and the solutions of the subsequent financial crisis in relation to the current U.S economic instability. Significance of Irving Fisher’s Debt-deflation Theory Irving Fisher’s debt-deflation theory was essential in the Great Depression era and in current U.S economic crisis because it clearly explains the factors that cause the debt and the resultant ripple effect on the economy. The U.S economy is essential in the global market because it is the largest trading partner in the world, which means its financial crisis affects all other foreign economies. The following are causes of deb t, stages of the financial crisis and proposed solutions as described and examined by Irving Fisher. a. Factors Led to the Great Depression i. Immediate Causes of Debt During the Great Depression, the two chief causes of debt for U.S citizens were over-investment, over-speculation and over-confidence (Fisher, 1933). Citizens, private sectors and the government borrowed money from banks, the federal government and other private financial lending institutions to conduct investments that they believed would result to higher returns and revenues. Many organizations and citizens wanted to invest borrowed money on new business ideas and receive abundant profits within a short time as compared to the ordinary investments that have moderate profits and interests. New inventions included building industries, railway lines, develop new resources, open real estates and new markets (Fisher, 1933). Financial lenders provided easy money, which led to over-borrowing. Most investors believe through speculation and over-confidence that they can borrow money at 6% and make profits of over 100% (Fisher, 1933). New industrial inventions and technology innovations prompted over-borrowing with the confidence of making a huge profit within a short period. Lucrative investment opportunities allowed people and organizations to have big debts. Major wars that the U.S initiated and

Proposing a Solution on Texting While Driving Essay

Proposing a Solution on Texting While Driving - Essay Example period. In other words, it amounts to something like a driver covering a length of football field at a speed of 55 mph without looking at the road. In another study carried out in 2008 it has been mentioned that over 800,000 Americans were texting or making calls, during the same year over 6,000 Americans were killed because of distracted driving and cell phone usage is one of the major reasons. Considering the extent of loss to the life that cell phone usage, specifically texting, there has been a Federal ban on texting while driving for commercial vehicle drivers. The defaulters may be subject to fines up to USD 2,750. But even after this ban the scene has not changed significantly. In order to propose a solution to address the issue of texting while driving it may be meaningful to understand the cause that leads the drivers to text. One of the national insurance agencies in America conducted a survey of over 1500 drives, nationwide. As per the findings of the survey, the key reaso n for people to use cell phones while driving is (Deleon) â€Å"social etiquette† demands that people be in constant contact with each other, so people feel pressured to responds immediately to text messages and phone calls.† This issue may be very difficult to be addressed comprehensively by adopting just one solution. Such issues can be addressed by two types of solutions, these are i) technology based solutions and ii) by bringing about a behavioral change in the actors concerned, in this particular case drivers are the key actors. In this paper, I would like to propose a solution that aims at kindling a behavioral change in the drivers. The justification for adopting such an approach is centered on the reason for which the people get urged for texting. The key audience in this proposal is the policy makers and society in general who are usually the drivers of social and behavioral change amongst people. If the audience, as I would propose in the next section, adopts the behavioral change strategies, I believe that there would be a considerable improvement in the statistics pertaining to texting while driving. It is also important to note that the proposal that I present is even though relevant for all drivers, but has specific benefits for commercial vehicle drivers. These are the ones exposed to high risks. The proposal is presented in the table below: Strategies of principle In order to induce a behavioral change amongst commercial drivers, I propose that the telecom companies should mandatorily send Statutory Warning about the dangers of texting while driving (TWD) before each text they send such as â€Å"TWD multiplies the risk of crash incidents by 23 times.† or â€Å"You might not be able to see the person whom you text if you text while driving.† Similar warnings may also be placed on strategic places inside the car. It is the job of the policy makers to ensure that each cellphone operators comply with this regulation. Con sequences of actions The consequences of this solution are anticipated to be: Subconscious learning by every cell user about the fallouts of TWD Advocate and Amplify the cause and risks of TWD Fall in the number of TWD accidents Analogies Advertisers, social marketers and policy makers have successfully applied Statutory Warnings in order to modify behaviors with respect to other unhealthy habits such as smoking, HIV/AIDS, toys using LASERS etc. Texting while driving is already being seen as a one of the unbending issue for all

Security Management in Maritime Organisations Case Study

Security Management in Maritime Organisations - Case Study Example TT Club aims at providing practical security measures which are limited to particular countries while the objective of the ISPS code is to establish an international maritime security framework. With the mutual coordination of contracting and local governments of shipping port facilities, the ISPS Code provides a complete theoretical solution to maritime possible threats and risks. One of the main reasons for comparing the TT Club manual with the ISPS code is to determine the extent to which the implementation of International Convention for the safety of Life at Sea adopted on December 12, 2002 made possible and followed. Other reasons to follow the ISPS Code are to list out all those possible threats which have never been considered before. The Club is a maritime Insurance company which provides all kinds of transport insurance to 80 countries of the world while the ISPS code targets all the shipping ports throughout the globe. TT Club provides pro-active determination of loss at some cost to its business members, but is limited to 80 countries. Another reason for comparing both the manuals is that the ISPS code is applied to all passenger and cargo ships (3.1) and one cannot ignore the fact that TT Club as an Insurance company is limited to provide maritime Insurance. Therefore it is obvious that TT Club would not take into account the hidden real world pro blems. Let us begin with the functional requirements of both the manuals. The TT Club manual is published with a view of preventing loss at the utmost. Moreover it serves as 'loss prevention guide'. The best evidence for calling it as 'loss prevention guide' is that it avoids security threats by avoiding computerised systems at the utmost. On the other hand, the ISPS Code is all about security of ships and ports and presents all techniques of preventing threats and risks and follows the functional requirements of the port (1.3). Ship Security Plan (SSP) containing Audits According to the ISPS Code 16.58, it is the responsibility of the SSP to conduct regular or updated audit reviews with the help of Company Security Officer (CSO) and Ship Security Officers (SSO) whereas the TT Club Manual suggests that Audit surveys are not limited to the responsibility of any particular person. However the main concern for both the manuals behind conducting audits i.e., ISPS code and TT Club Manual is to assess security at all levels to determine vulnerable access points. According to Security Level 1 of Section 7 of the Ship Security which sets appropriate controlling, monitoring and supervising access to different areas of the ship including deck, cargo and ship's stores, TT Club manual takes hold and observes all access points of the ship. These include all kinds of used and unused gates, access to the port facilities, and physical and logical security threats. Security Levels of the ISPS Code The security levels are set by priorities of their associated risks. Like level 1 is the normal level which is usually followed by the ships and ports. Security level 2 is applied in a condition which in addition to the threat exposure is experiencing a critical risk. Therefore the level is applied as long as the risk is there. Similarly level 3 entail exceptional threats, which usually do not occur in

Economists Essay Example | Topics and Well Written Essays - 1750 words

Economists - Essay Example Fisher was born in 1876 and received his Business Administration degree and Ph. D in Yale University. As the professor of economics and mathematics at Yale University, Irving Fisher developed extensive concepts concerning money supply and price fluctuations using mathematical evidence (Allen, 1977). Fisher was among the most accurate and leading economists of the 20th century. He used his acquired knowledge and skills from Yale to observe and analyze dramatic and complex macroeconomic events during his era. Fisher also proposed appropriate solutions to the Great Depression that crippled the U.S economy and other aspects of life. The crash of the stock market in the U.S in 1929 and consequential falling of stocks and goods’ prices prompted Fisher to revise his economic theories on price fluctuations, debt and the depression caused by these factors (Mendoza, 2009). Fisher formulated a debt-deflation theory and named the financial crisis, the Great Depression, which is currently used to relate to the economic recession in the U.S.The debt-deflation theory indicates that consumers borrow money for investment on speculation and imagine profits, which leads to losses, debt, deflation and pessimism. Thesis Statement: Irving Fisher has a significance impact on the prevailing U.S economy through his debt-deflation theory, which examines the causes of debt, the deflation and the solutions of the subsequent financial crisis in relation to the current U.S economic instability. Significance of Irving Fisher’s Debt-deflation Theory Irving Fisher’s debt-deflation theory was essential in the Great Depression era and in current U.S economic crisis because it clearly explains the factors that cause the debt and the resultant ripple effect on the economy. The U.S economy is essential in the global market because it is the largest trading partner in the world, which means its financial crisis affects all other foreign economies. The following are causes of deb t, stages of the financial crisis and proposed solutions as described and examined by Irving Fisher. a. Factors Led to the Great Depression i. Immediate Causes of Debt During the Great Depression, the two chief causes of debt for U.S citizens were over-investment, over-speculation and over-confidence (Fisher, 1933). Citizens, private sectors and the government borrowed money from banks, the federal government and other private financial lending institutions to conduct investments that they believed would result to higher returns and revenues. Many organizations and citizens wanted to invest borrowed money on new business ideas and receive abundant profits within a short time as compared to the ordinary investments that have moderate profits and interests. New inventions included building industries, railway lines, develop new resources, open real estates and new markets (Fisher, 1933). Financial lenders provided easy money, which led to over-borrowing. Most investors believe through speculation and over-confidence that they can borrow money at 6% and make profits of over 100% (Fisher, 1933). New industrial inventions and technology innovations prompted over-borrowing with the confidence of making a huge profit within a short period. Lucrative investment opportunities allowed people and organizations to have big debts. Major wars that the U.S initiated and

Security Management in Maritime Organisations Case Study

Security Management in Maritime Organisations - Case Study Example TT Club aims at providing practical security measures which are limited to particular countries while the objective of the ISPS code is to establish an international maritime security framework. With the mutual coordination of contracting and local governments of shipping port facilities, the ISPS Code provides a complete theoretical solution to maritime possible threats and risks. One of the main reasons for comparing the TT Club manual with the ISPS code is to determine the extent to which the implementation of International Convention for the safety of Life at Sea adopted on December 12, 2002 made possible and followed. Other reasons to follow the ISPS Code are to list out all those possible threats which have never been considered before. The Club is a maritime Insurance company which provides all kinds of transport insurance to 80 countries of the world while the ISPS code targets all the shipping ports throughout the globe. TT Club provides pro-active determination of loss at some cost to its business members, but is limited to 80 countries. Another reason for comparing both the manuals is that the ISPS code is applied to all passenger and cargo ships (3.1) and one cannot ignore the fact that TT Club as an Insurance company is limited to provide maritime Insurance. Therefore it is obvious that TT Club would not take into account the hidden real world pro blems. Let us begin with the functional requirements of both the manuals. The TT Club manual is published with a view of preventing loss at the utmost. Moreover it serves as 'loss prevention guide'. The best evidence for calling it as 'loss prevention guide' is that it avoids security threats by avoiding computerised systems at the utmost. On the other hand, the ISPS Code is all about security of ships and ports and presents all techniques of preventing threats and risks and follows the functional requirements of the port (1.3). Ship Security Plan (SSP) containing Audits According to the ISPS Code 16.58, it is the responsibility of the SSP to conduct regular or updated audit reviews with the help of Company Security Officer (CSO) and Ship Security Officers (SSO) whereas the TT Club Manual suggests that Audit surveys are not limited to the responsibility of any particular person. However the main concern for both the manuals behind conducting audits i.e., ISPS code and TT Club Manual is to assess security at all levels to determine vulnerable access points. According to Security Level 1 of Section 7 of the Ship Security which sets appropriate controlling, monitoring and supervising access to different areas of the ship including deck, cargo and ship's stores, TT Club manual takes hold and observes all access points of the ship. These include all kinds of used and unused gates, access to the port facilities, and physical and logical security threats. Security Levels of the ISPS Code The security levels are set by priorities of their associated risks. Like level 1 is the normal level which is usually followed by the ships and ports. Security level 2 is applied in a condition which in addition to the threat exposure is experiencing a critical risk. Therefore the level is applied as long as the risk is there. Similarly level 3 entail exceptional threats, which usually do not occur in

Good vs Evil - English Essay Essay Example for Free

Good vs Evil English Essay Essay There is neither a definite good nor evil, but only the grey area in-between. The Japanese proverb of Yin and Yang states that in all good there is some evil and in all evil there is dome good thus without good there can be no evil and without evil there can be no good. Evil was once described to me as ‘The inability to feel sympathy’ and at first I agreed with this analogy but upon further thought and deliberation I decided that it was not entirely true, for if evil is the inability to feel sympathy then that makes all acts of sympathy good. However, that analogy would make an act, one that almost the entire world agrees is purely evil, an act of good. The act in question is the destruction of the twin towers of 9/11, because the perpetrators were sympathetic. They were sympathetic towards their country and their people who, contrary to popular knowledge, had already been invaded and were being oppressed by the USA who were trying to secure their oil reserves. There is no way to moderate universally what is considered good and evil. Every man believes in some way that their own actions are just and righteous whilst also believing that their enemy’s actions are acts of evil purely because they oppose his own wishes thus making all man good in his own eyes yet evil in the eyes of those he opposes. Each and every one of us must decide for ourselves what is good and what is evil, and yet we must still conform to the â€Å"norms† of society. The definition of what is right and what is wrong may come from many different places. Some blindly accept what society tells then to where others question society and still others actively oppose society’s views. Some get their definition from the relative religious scripture and follow that without question whereas others merely see it as a general guideline whereupon to base their actions. I believe that the concepts of good and bad, righteousness and evil, just and unjust exist purely to justify man’s actions and that they have and will continue to change to fit the person whose action requires justifying. Alas, these unflinching opposing forces are bound to perpetually clash as they strive for a balance. For without either life as we know it could never exist, perhaps life at all could not exist.

High Performance Liquid Chromatography (HPLC) 214

High Performance Liquid Chromatography (HPLC) 214 Introduction High performance liquid chromatography 214 is the most widely used of all of the analytical separation techniques. The reasons for the popularity of the method is its sensitivity, ready adaptability to accurate quantitative determinations, suitability for separating non-volatile species or thermally fragile ones, wide spread applicability to substance that are of prime interest to industry, many fields of science and the public. The applications of chromatography have grown explosively in the last fifty years owing not only to the development of several new types of chromatographic techniques but also to the growing need by scientist for better methods for characterizing complex mixtures. General methodology for the development of new HPLC methods 215-228 HPLC method development follows the series of steps summarized below. Information on sample, objective of separation. Need for special HPLC procedure, sample pretreatment etc. Choice of detector and detector settings. Choosing LC method, preliminary run, estimation of best separation conditions. Optimization of separation conditions. Check for problems or requirement for special procedure. a) Recovery of purified material   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   b) Quantitative calibration  Ã‚  Ã‚  Ã‚  Ã‚   c) Qualitative method Validate method for routine laboratory use. A good method development strategy should require only as many experimental runs as are necessary to achieve the desired final result. Finally, method development should be simple as possible, yet it should allow the use of sophisticated tools such as computer modeling if these are available. Before the beginning of method development, it is necessary to review what is known about the sample in order to define the goals of separation. The kinds of sample related information that can be important are summarized in Table-7.1. Table-8.1 Important information concerning sample composition and properties Number   of compounds present in the sample Chemical structures of components Molecular weights of compounds PKa values of compounds UV spectra of compounds Concentration range of various compounds in samples of interest Sample solubility   Ã‚   The chemical composition of the sample can provide valuable clues for the best choice of initial conditions for an HPLC separation. Objectives of separation The objectives of HPLC separation need to be specified clearly include. The use of HPLC to isolate purified sample components for spectral identification or quantitative analysis. It may be necessary to separate all degradants or impurities from a product for reliable content assay. In quantitative analysis, the required levels of accuracy and precision should be known (a precision of  ± 1 to 2% is usually achievable). Whether a single HPLC procedure is sufficient for raw material or one or more formulations and / or different procedures are desired for the analysis of formulations? When the number of samples for analysis at one time is greater than 10, a run time of less than 20 min. will be oftenly important. Knowledge on the desired HPLC equipment, experience and academic training the operators have. Sample pretreatment and detection Samples for analysis come in various forms such as: Solutions ready for injections. Solutions that require dilution, buffering, addition of an internal standard or other volumetric manipulation. Solids that must first be dissolved or extracted. Samples that require pretreatment to remove interference and/or protect the column or equipment from damage. Most samples for HPLC analysis require weighing and / or volumetric dilution before injection. Best results are often obtained when the composition of the sample solvent is close to that of the mobile phase since this minimizes baseline upset and other problems. Some samples require a partial separation ( pretreatment) prior to HPLC, because of need to remove interference, concentrate sample analytes or eliminate â€Å"column killer†. In many cases the development of an adequate sample pretreatment can be challenging than achieving a good HPLC separation. The detector selected should sense all sample components of interest. Variable-wavelength ultraviolet (UV) detectors normally are the first choice, because of their convenience and applicability for most samples. For this reason information on the UV spectra can be an important aid for method development. When the UV response of the sample is inadequate, other detectors are available (flourescence, electrochemical, PDA etc.) or the sample can be derivatized for enhanced detection. Developing the method for the separation Selecting an HPLC method and initial conditions If HPLC is chosen for the separation, the next step is to classify the sample as regular or special. Regular samples means typical mixtures of small molecules (    Table-8.2 Handling of special sample Sample Requirements Inorganic ions Detection is primary problems; use ion chromatography Isomers Some isomers can be separated by reversed-phase HPLC and are then classified as regular samples; better separations of isomers are obtainable using either (1) normal-phase HPLC or (2) reversed-phase separations with cyclodextrin-silica columns. Enantiomers These compounds require â€Å"chiral† conditions for their separations. Biological Several factors make samples or this kind â€Å"special†; molecular conformation, polar functionality and a wide range of hydrophobicity. Macromolecules â€Å"Big† molecules require column packing with large pores  Ã‚  (>> 10-nm diameters); in addition, biological molecules require special conditions as noted above. Table-8.3 Preferred experimental conditions for the initial HPLC separation Separation variable Preferred initial choice Column Dimensions (length, ID) 15 x 0.46 cm Particle size 5 mma Stationary phase C8 or C18 Mobile phase Solvent A and B Buffer-acetonitrile % B 80-100%b Buffer (compound, pH, concentration) 25mM potassium phosphate 2.0 Additives (e.g., amine modifiers, ion pair reagents) Do not use initially Flow rate 1.5–2.0 ml/min Temperature 35-45 ºC Sample Size Volumed >25 mL Weightd B : Polar solvent  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   a 3.5 mm particles are an alternative using a 7.5 cm column b For an initial isocratic run; an initial gradient run is preferred. c No buffer required for neutral samples; for pH d Smaller values required for smaller-volume columns (e.g., 7.50.46-cm, 3.5-mm column). Table-8.4 Physical properties of silica supports for some C 18 columns Column (mL/mL) Pore diameter (nm) Surface area (m2/g) Percent Porosity Hypersil ODS 12 170 57 LiChrosorb C18 10 355 71 Novapak C18 6 N/Aa N/Aa Nucleosil C18 10 350 69` Symmetry C18 10 335 66 Zorbax ODS 6 300 55 Zorbax Rx, SB, XDB 8 180 50 a N/A : Not available On the basis of the initial exploratory run isocratic or gradient elution can be selected as most suitable. If typical reversed-phase conditions provide insufficient sample retention, suggesting the use of either ion pair on normal phase HPLC. Alternatively, the sample may be strongly retained with 100% acetonitrile as mobile phase, suggesting the use of non-aqueous reversed-phase (NARP) chromatography or normal phase HPLC. Some characteristics of reversed-phase and other HPLC methods are summarized below. Table-8.5 Characteristics of primary HPLC methods Method / description/ columns Preferred method Reversed-phase HPLC Uses water – organic mobile phase Columns: C18 (ODS), C8, phenyl, trimethylsilyl (TMS), Cyano First choice for most samples, especially neutral or non-ionisable compounds that dissolve in water-organic mixtures Ion-pair HPLC Uses water-organic mobile phase a buffer to control pH and an ion pair reagent. Column : C18, C8, cyano. Acceptable choice for ionic or ionizable compounds, especially bases or cations. Normal phase HPLC Uses mixtures of organic solvents as mobile phase Columns: Cyano, diol, amino and silica. Good second choice when reversed-phase or ion-pair HPLC is ineffective, first choice for lipophilic samples that do not dissolve well in water-organic mixtures, first choice for mixtures of isomers and for preparative-scale HPLC (silica best) Getting started on method development One approach is to use an isocratic mobile phase of some average solvent strength (e.g., 50%) organic solvent. A better alternative is to use a very strong mobile phase with (80-100% B), then reduce %B as necessary. The initial separation with 100%B results in rapid elution of the entire sample, but few groups will separate. Decreasing solvent strength shows the rapid separation of all components with a much longer run time, with a broadening of later bands and reduced detection sensitivity. Improving the separation and repeatable separation Generally the chromatographers will consider several aspects of the separation, as summarized in Table-8.6. Table-8.6 Objectives of separation in HPLC method development Objectivesa Comment Resolution Precise and rugged quantitative analysis requires that Rs be greater than 1.5. Separation time Quantitation   Ã‚ £ 2% (1 SD) for assays;  £ 5% for less-demanding analysis;  £15% for trace analysis. Pressure Peak height Narrow peaks are desirable for large signal / noise ratios Solvent consumption   Minimum mobile-phase use per run is desirable. a Roughly in order of decreasing importance but may vary with analysis requirements. Separation or resolution is a primary requirement in quantitative HPLC. The resolution (Rs) value should be maximum (Rs>1.5) favours maximum precision. Resolution usually degrades during the life of the column and can vary from day to day with minor fluctuations in separation conditions. Therefore, values of Rs = 2 or greater should be the goal during method development for simple mixtures. Such resolution will favour both improved assay precision and greater method ruggedness. Some HPLC assays do not require base line separation of the compounds of interest (qualitative analysis). In such cases only enough separation of individual components is required to provide characteristic retention times for peak identification. The time required for a separation (run time = retention time for base band) should be as short as possible and the total time spent on method development is reasonable (runtimes 5 to 10 minutes are desirable). Conditions for the final HPLC method should be selected so that the operating pressure with a new column does not exceed 170 bar (2500 psi) and upper pressure limit below 2000 psi is desirable. There are two reasons for that pressure limit, despite the fact that most HPLC equipment can be operated at much higher pressures. First, during the life of a column, the back pressure may rise by a factor of as much as 2 due to the gradual plugging of the column by particular matter. Second, at lower pressures When dealing with more challenging samples or if the goals of separation are particularly stringent, a large number of method development runs may be required to achieve acceptable separation. Repeatable separation As the experimental runs described above are being carried out, it is important to confirm that each chromatogram can be repeated. When changing conditions (mobile phase, column, and temperature) between method development experiments, enough time must elapse for the column to come into equilibrium with a new mobile phase and temperature. Usually column equilibration is achieved after passage of 10 to 20 column volumes of the new mobile phase through the column. However, this should be confirmed by carrying out a repeat experiment under the same conditions. When constant retention times are observed in two such back-to-back repeat experiments ( ± 0.5% or better), it can be assumed that the column is equilibrated and the experiments are repeatable. Completing the HPLC method development The final procedure should meet all the objectives that were defined at the beginning of method development. The method should also be robust in routine operation and usable by all laboratories and personnel for which it is intended. Quantitation and method validation One of the strengths of HPLC is that is an excellent quantitative analytical technique. HPLC can be used for the quantitation of the primary or major component of a sample (including pure samples) for mixture of many compounds at intermediate concentrations and for the assessment of trace impurity concentrations in matrix. Method validation, according to the United States Pharmacopoeia (USP), is performed to ensure that an analytical methodology is accurate, specific, reproducible and rugged over the specified range that an analyte will be analysed. Method validation provides an assurance of reliability during normal use and is sometimes described as the process of providing documented evidence that the method does what it is intended to do. According to USP, the method validation involves eight steps as given below. Precision Accuracy Limit of detection Limit of quantitation Specificity Linearity and range Ruggedness Robustness Precision and accuracy: Already discussed in chapter-1. Linearity The linearity of the method is a measure of how well a calibration plot of response v/s concentration approximates a straight line, or how well the data fit to the linear equation. Y = aX + b Where ‘Y’ is the response, ‘X’ is the concentration, ‘a’ is the slope and ‘b’ is the intercept of a line fit to the data. Ideally, a linear relationship is preferred (b = 0) because it is more precise, easier for calculations and can be defined with fewer standards. Also, UV detector response for a dilute sample is expected to follow Beer’s law and be linear. Therefore, a linear calibration gives evidence that the system is performing properly throughout the concentration range of interest. Generally in HPLC, if we are using internal standard, then the linearity plot is to be drawn by taking concentration of the analyte on x-axis and the ratio of area under the curve (AUC) of analyte to AUC of internal standard (IS) on y-axis. The resulting plot slope, intercept and correlation coefficient provide the desired information on linearity. A linearity correlation coefficient above 0.999 is acceptable for most methods. Limit of detection (LOD) The limit of detection (LOD) is the smallest concentration that can be detected reliably. The LOD represents the concentration of analyte that would yield a signal-to-noise (S/N) ratio of 3. Limit of quantitation (LOQ) The LOQ is the concentration that can be quantitated reliably with a specified level of accuracy and precision. The LOQ represents the concentration of analyte that would yield a signal-to-noise ratio of 10. LOD and LOQ can be determined by using the following expressions. LOD  Ã‚  Ã‚   =  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   3 X N / B LOQ  Ã‚  Ã‚   =  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   10 X N / B Where N is the noise estimate, is the standard deviation of the peak area ratio of analyte to IS (5 injections) of the drugs. B is the slope of the corresponding calibration curve. The LOD and LOQ values determined during method validation are affected by the separation conditions, columns, reagents and especially instrumentation and data systems. Ruggedness Method ruggedness is defined as the reproducibility of results when the method is performed under actual use conditions. This includes different analysts, laboratories, columns, instruments, sources, chemicals, solvents etc. method ruggedness may not be known when a method is first developed, but insight is obtained during subsequent use of that method. Robustness The concept of robustness of an analytical procedure has been defined by the ICH as â€Å" a measure of its capacity to remain unaffected by small, but deliberate variations in method parameters†. The robustness of a method is the ability to remain unaffected by small changes in parameters such as pH of the mobile phase, temperature, percentage of organic solvent and buffer concentration etc. to determine robustness of the method experimental conditions were purposely altered and chromatographic characteristics were evaluated. To study the pH effect on the retention (K1) of the drug, buffer pH is to be changed by 0.2 units. At certain point, retention will increase at any pH above and below of the pH unit. The effect of temperature on the retention characteristics (K1) of the drug is to be studied by changing the temperature in steps 2 ºC from room temperature to 80 ºC and see the effect of temperature on the resolution and peak shape. Effect of percentage organic strength on retention is to be studied by varying the percentage of organic solvents like acetonitrile, methanol etc. from 0 to 2% while the other mobile phase contents are held constant and observe the K1. At certain point decreases in K1 observed with increase in the level of organic solvent. Effect of buffer concentration should be checked at three concentration levels i.e. 0.025 M, 0.05 M and 0.1 M and observe retention time and resolution. Stability To generate reproducible and reliable results, the samples, standards and reagents used for the HPLC method must be stable for a reasonable time (e.g., One day, one week, one month, depending on the need). For example, the analysis of even a single sample may require 10 or more chromatographic runs to determine system suitability, including standard concentrations to create a working analytical curve and duplicate or triplicate injections of the sample to be assayed. Therefore, a few hours of standard and sample solution stability can be required even for a short (10 min.) separation. When more than one sample is analyzed, automated, over night runs often are performed for better laboratory efficiency. Typically, 24 hours stability is desired for all solutions and reagents that need to be prepared for each analysis. Mobile phases should be chosen to avoid stability problems, especially the use of amine additives or specific solvents. For example, mobile phase containing THF (tetra hydrofuran) are known to be susceptible to oxidation, therefore, the mobile phase should be prepared daily with fresh THF. Some buffered mobile phases cause problems for example, phosphate and acetate provide good media for microbial growth. Sodium oxide (0.1%) is often added to the mobile phase buffer to inhibit such growth, adding more than 5% of organic solvent is also effective. Long term column stability is critical for method ruggedness. Even the best HPLC column will eventually degrade and lose its initial performance, often as a function of the number of samples injected. System suitability System suitability experiments can be defined as tests to ensure that the method can generate results of acceptable accuracy and precision. The requirements for system suitability are usually developed after method development and validation have been completed. The criteria selected will be based on the actual performance of the method as determined during its validation. For example, if sample retention times forms part of the system suitability criteria, their variation (SD) during validation can be determined, system suitability might then require that retention times fall within a  ±3 SD range during routine performance of the method. The USP (2000) defines parameters that can be used to determine system suitability prior to analysis. These parameters include plate number (N), tailing factor, k and / or a, resolution (Rs) and relative standard deviation (RSD) of peak height or peak area for respective injections. The RSD of peak height or area of five injections of standard solution is normally accepted as one of the standard criteria. For an assay method of a major component, the RSD should typically be less than 1% for these five respective injections. The plate number and / or tailing factor are used if the run contains only one peak. For chromatographic separations with more than one peak, such as an internal standard assay or an impurity method, expected to contain many peaks, some measure of separations such as Rs is recommended. Reproducibility of tR or k value for a specific compound also defines system performance. The column performance can be defined in terms of column plate number ‘N’ is defined by N = 5.54 (tR / W ½)2 Where ‘tR’ is the retention time of the peak and ‘W ½Ã¢â‚¬â„¢ is the width of the peak at half peak height. The resolution of two adjacent peaks can be calculated by using the formula Rs = 1.18 (t2-t1) / W0.5.1 +W0.5.2 Where ‘t1’ and ‘t2’ are retention times of the adjacent peaks and W0.5.1 and W0.5.2 are the width of the peaks at half height. Rs = 2.0 or greater is a desirable target for method development. The retention factor k is given by the equation. k = (tR – t0) / t0 where ‘tR’ is the band retention time and t0 is the column dead time. The peak symmetry can be represented in terms of peak asymmetry factor and peak tailing factor, which can be calculated by using the following formula. Peak asymmetry factor = B /A Where ‘B’ is the distance at 50% peak height between leading edge to the perpendicular drawn from the peak maxima and ‘A’ is the width of the peak at half height. According to USP (2000) peak tailing factor can be calculated by using the formula T = W0.05 / 2f Where â€Å"W0.05† is the width of the peak at 5% height and â€Å"f† is the distance from the peak maximum to the leading edge of the peak, the distance being measured at a point 50% of the peak height from the base line. High Performance Liquid Chromatography (HPLC) 214 High Performance Liquid Chromatography (HPLC) 214 Introduction High performance liquid chromatography 214 is the most widely used of all of the analytical separation techniques. The reasons for the popularity of the method is its sensitivity, ready adaptability to accurate quantitative determinations, suitability for separating non-volatile species or thermally fragile ones, wide spread applicability to substance that are of prime interest to industry, many fields of science and the public. The applications of chromatography have grown explosively in the last fifty years owing not only to the development of several new types of chromatographic techniques but also to the growing need by scientist for better methods for characterizing complex mixtures. General methodology for the development of new HPLC methods 215-228 HPLC method development follows the series of steps summarized below. Information on sample, objective of separation. Need for special HPLC procedure, sample pretreatment etc. Choice of detector and detector settings. Choosing LC method, preliminary run, estimation of best separation conditions. Optimization of separation conditions. Check for problems or requirement for special procedure. a) Recovery of purified material   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   b) Quantitative calibration  Ã‚  Ã‚  Ã‚  Ã‚   c) Qualitative method Validate method for routine laboratory use. A good method development strategy should require only as many experimental runs as are necessary to achieve the desired final result. Finally, method development should be simple as possible, yet it should allow the use of sophisticated tools such as computer modeling if these are available. Before the beginning of method development, it is necessary to review what is known about the sample in order to define the goals of separation. The kinds of sample related information that can be important are summarized in Table-7.1. Table-8.1 Important information concerning sample composition and properties Number   of compounds present in the sample Chemical structures of components Molecular weights of compounds PKa values of compounds UV spectra of compounds Concentration range of various compounds in samples of interest Sample solubility   Ã‚   The chemical composition of the sample can provide valuable clues for the best choice of initial conditions for an HPLC separation. Objectives of separation The objectives of HPLC separation need to be specified clearly include. The use of HPLC to isolate purified sample components for spectral identification or quantitative analysis. It may be necessary to separate all degradants or impurities from a product for reliable content assay. In quantitative analysis, the required levels of accuracy and precision should be known (a precision of  ± 1 to 2% is usually achievable). Whether a single HPLC procedure is sufficient for raw material or one or more formulations and / or different procedures are desired for the analysis of formulations? When the number of samples for analysis at one time is greater than 10, a run time of less than 20 min. will be oftenly important. Knowledge on the desired HPLC equipment, experience and academic training the operators have. Sample pretreatment and detection Samples for analysis come in various forms such as: Solutions ready for injections. Solutions that require dilution, buffering, addition of an internal standard or other volumetric manipulation. Solids that must first be dissolved or extracted. Samples that require pretreatment to remove interference and/or protect the column or equipment from damage. Most samples for HPLC analysis require weighing and / or volumetric dilution before injection. Best results are often obtained when the composition of the sample solvent is close to that of the mobile phase since this minimizes baseline upset and other problems. Some samples require a partial separation ( pretreatment) prior to HPLC, because of need to remove interference, concentrate sample analytes or eliminate â€Å"column killer†. In many cases the development of an adequate sample pretreatment can be challenging than achieving a good HPLC separation. The detector selected should sense all sample components of interest. Variable-wavelength ultraviolet (UV) detectors normally are the first choice, because of their convenience and applicability for most samples. For this reason information on the UV spectra can be an important aid for method development. When the UV response of the sample is inadequate, other detectors are available (flourescence, electrochemical, PDA etc.) or the sample can be derivatized for enhanced detection. Developing the method for the separation Selecting an HPLC method and initial conditions If HPLC is chosen for the separation, the next step is to classify the sample as regular or special. Regular samples means typical mixtures of small molecules (    Table-8.2 Handling of special sample Sample Requirements Inorganic ions Detection is primary problems; use ion chromatography Isomers Some isomers can be separated by reversed-phase HPLC and are then classified as regular samples; better separations of isomers are obtainable using either (1) normal-phase HPLC or (2) reversed-phase separations with cyclodextrin-silica columns. Enantiomers These compounds require â€Å"chiral† conditions for their separations. Biological Several factors make samples or this kind â€Å"special†; molecular conformation, polar functionality and a wide range of hydrophobicity. Macromolecules â€Å"Big† molecules require column packing with large pores  Ã‚  (>> 10-nm diameters); in addition, biological molecules require special conditions as noted above. Table-8.3 Preferred experimental conditions for the initial HPLC separation Separation variable Preferred initial choice Column Dimensions (length, ID) 15 x 0.46 cm Particle size 5 mma Stationary phase C8 or C18 Mobile phase Solvent A and B Buffer-acetonitrile % B 80-100%b Buffer (compound, pH, concentration) 25mM potassium phosphate 2.0 Additives (e.g., amine modifiers, ion pair reagents) Do not use initially Flow rate 1.5–2.0 ml/min Temperature 35-45 ºC Sample Size Volumed >25 mL Weightd B : Polar solvent  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   a 3.5 mm particles are an alternative using a 7.5 cm column b For an initial isocratic run; an initial gradient run is preferred. c No buffer required for neutral samples; for pH d Smaller values required for smaller-volume columns (e.g., 7.50.46-cm, 3.5-mm column). Table-8.4 Physical properties of silica supports for some C 18 columns Column (mL/mL) Pore diameter (nm) Surface area (m2/g) Percent Porosity Hypersil ODS 12 170 57 LiChrosorb C18 10 355 71 Novapak C18 6 N/Aa N/Aa Nucleosil C18 10 350 69` Symmetry C18 10 335 66 Zorbax ODS 6 300 55 Zorbax Rx, SB, XDB 8 180 50 a N/A : Not available On the basis of the initial exploratory run isocratic or gradient elution can be selected as most suitable. If typical reversed-phase conditions provide insufficient sample retention, suggesting the use of either ion pair on normal phase HPLC. Alternatively, the sample may be strongly retained with 100% acetonitrile as mobile phase, suggesting the use of non-aqueous reversed-phase (NARP) chromatography or normal phase HPLC. Some characteristics of reversed-phase and other HPLC methods are summarized below. Table-8.5 Characteristics of primary HPLC methods Method / description/ columns Preferred method Reversed-phase HPLC Uses water – organic mobile phase Columns: C18 (ODS), C8, phenyl, trimethylsilyl (TMS), Cyano First choice for most samples, especially neutral or non-ionisable compounds that dissolve in water-organic mixtures Ion-pair HPLC Uses water-organic mobile phase a buffer to control pH and an ion pair reagent. Column : C18, C8, cyano. Acceptable choice for ionic or ionizable compounds, especially bases or cations. Normal phase HPLC Uses mixtures of organic solvents as mobile phase Columns: Cyano, diol, amino and silica. Good second choice when reversed-phase or ion-pair HPLC is ineffective, first choice for lipophilic samples that do not dissolve well in water-organic mixtures, first choice for mixtures of isomers and for preparative-scale HPLC (silica best) Getting started on method development One approach is to use an isocratic mobile phase of some average solvent strength (e.g., 50%) organic solvent. A better alternative is to use a very strong mobile phase with (80-100% B), then reduce %B as necessary. The initial separation with 100%B results in rapid elution of the entire sample, but few groups will separate. Decreasing solvent strength shows the rapid separation of all components with a much longer run time, with a broadening of later bands and reduced detection sensitivity. Improving the separation and repeatable separation Generally the chromatographers will consider several aspects of the separation, as summarized in Table-8.6. Table-8.6 Objectives of separation in HPLC method development Objectivesa Comment Resolution Precise and rugged quantitative analysis requires that Rs be greater than 1.5. Separation time Quantitation   Ã‚ £ 2% (1 SD) for assays;  £ 5% for less-demanding analysis;  £15% for trace analysis. Pressure Peak height Narrow peaks are desirable for large signal / noise ratios Solvent consumption   Minimum mobile-phase use per run is desirable. a Roughly in order of decreasing importance but may vary with analysis requirements. Separation or resolution is a primary requirement in quantitative HPLC. The resolution (Rs) value should be maximum (Rs>1.5) favours maximum precision. Resolution usually degrades during the life of the column and can vary from day to day with minor fluctuations in separation conditions. Therefore, values of Rs = 2 or greater should be the goal during method development for simple mixtures. Such resolution will favour both improved assay precision and greater method ruggedness. Some HPLC assays do not require base line separation of the compounds of interest (qualitative analysis). In such cases only enough separation of individual components is required to provide characteristic retention times for peak identification. The time required for a separation (run time = retention time for base band) should be as short as possible and the total time spent on method development is reasonable (runtimes 5 to 10 minutes are desirable). Conditions for the final HPLC method should be selected so that the operating pressure with a new column does not exceed 170 bar (2500 psi) and upper pressure limit below 2000 psi is desirable. There are two reasons for that pressure limit, despite the fact that most HPLC equipment can be operated at much higher pressures. First, during the life of a column, the back pressure may rise by a factor of as much as 2 due to the gradual plugging of the column by particular matter. Second, at lower pressures When dealing with more challenging samples or if the goals of separation are particularly stringent, a large number of method development runs may be required to achieve acceptable separation. Repeatable separation As the experimental runs described above are being carried out, it is important to confirm that each chromatogram can be repeated. When changing conditions (mobile phase, column, and temperature) between method development experiments, enough time must elapse for the column to come into equilibrium with a new mobile phase and temperature. Usually column equilibration is achieved after passage of 10 to 20 column volumes of the new mobile phase through the column. However, this should be confirmed by carrying out a repeat experiment under the same conditions. When constant retention times are observed in two such back-to-back repeat experiments ( ± 0.5% or better), it can be assumed that the column is equilibrated and the experiments are repeatable. Completing the HPLC method development The final procedure should meet all the objectives that were defined at the beginning of method development. The method should also be robust in routine operation and usable by all laboratories and personnel for which it is intended. Quantitation and method validation One of the strengths of HPLC is that is an excellent quantitative analytical technique. HPLC can be used for the quantitation of the primary or major component of a sample (including pure samples) for mixture of many compounds at intermediate concentrations and for the assessment of trace impurity concentrations in matrix. Method validation, according to the United States Pharmacopoeia (USP), is performed to ensure that an analytical methodology is accurate, specific, reproducible and rugged over the specified range that an analyte will be analysed. Method validation provides an assurance of reliability during normal use and is sometimes described as the process of providing documented evidence that the method does what it is intended to do. According to USP, the method validation involves eight steps as given below. Precision Accuracy Limit of detection Limit of quantitation Specificity Linearity and range Ruggedness Robustness Precision and accuracy: Already discussed in chapter-1. Linearity The linearity of the method is a measure of how well a calibration plot of response v/s concentration approximates a straight line, or how well the data fit to the linear equation. Y = aX + b Where ‘Y’ is the response, ‘X’ is the concentration, ‘a’ is the slope and ‘b’ is the intercept of a line fit to the data. Ideally, a linear relationship is preferred (b = 0) because it is more precise, easier for calculations and can be defined with fewer standards. Also, UV detector response for a dilute sample is expected to follow Beer’s law and be linear. Therefore, a linear calibration gives evidence that the system is performing properly throughout the concentration range of interest. Generally in HPLC, if we are using internal standard, then the linearity plot is to be drawn by taking concentration of the analyte on x-axis and the ratio of area under the curve (AUC) of analyte to AUC of internal standard (IS) on y-axis. The resulting plot slope, intercept and correlation coefficient provide the desired information on linearity. A linearity correlation coefficient above 0.999 is acceptable for most methods. Limit of detection (LOD) The limit of detection (LOD) is the smallest concentration that can be detected reliably. The LOD represents the concentration of analyte that would yield a signal-to-noise (S/N) ratio of 3. Limit of quantitation (LOQ) The LOQ is the concentration that can be quantitated reliably with a specified level of accuracy and precision. The LOQ represents the concentration of analyte that would yield a signal-to-noise ratio of 10. LOD and LOQ can be determined by using the following expressions. LOD  Ã‚  Ã‚   =  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   3 X N / B LOQ  Ã‚  Ã‚   =  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   10 X N / B Where N is the noise estimate, is the standard deviation of the peak area ratio of analyte to IS (5 injections) of the drugs. B is the slope of the corresponding calibration curve. The LOD and LOQ values determined during method validation are affected by the separation conditions, columns, reagents and especially instrumentation and data systems. Ruggedness Method ruggedness is defined as the reproducibility of results when the method is performed under actual use conditions. This includes different analysts, laboratories, columns, instruments, sources, chemicals, solvents etc. method ruggedness may not be known when a method is first developed, but insight is obtained during subsequent use of that method. Robustness The concept of robustness of an analytical procedure has been defined by the ICH as â€Å" a measure of its capacity to remain unaffected by small, but deliberate variations in method parameters†. The robustness of a method is the ability to remain unaffected by small changes in parameters such as pH of the mobile phase, temperature, percentage of organic solvent and buffer concentration etc. to determine robustness of the method experimental conditions were purposely altered and chromatographic characteristics were evaluated. To study the pH effect on the retention (K1) of the drug, buffer pH is to be changed by 0.2 units. At certain point, retention will increase at any pH above and below of the pH unit. The effect of temperature on the retention characteristics (K1) of the drug is to be studied by changing the temperature in steps 2 ºC from room temperature to 80 ºC and see the effect of temperature on the resolution and peak shape. Effect of percentage organic strength on retention is to be studied by varying the percentage of organic solvents like acetonitrile, methanol etc. from 0 to 2% while the other mobile phase contents are held constant and observe the K1. At certain point decreases in K1 observed with increase in the level of organic solvent. Effect of buffer concentration should be checked at three concentration levels i.e. 0.025 M, 0.05 M and 0.1 M and observe retention time and resolution. Stability To generate reproducible and reliable results, the samples, standards and reagents used for the HPLC method must be stable for a reasonable time (e.g., One day, one week, one month, depending on the need). For example, the analysis of even a single sample may require 10 or more chromatographic runs to determine system suitability, including standard concentrations to create a working analytical curve and duplicate or triplicate injections of the sample to be assayed. Therefore, a few hours of standard and sample solution stability can be required even for a short (10 min.) separation. When more than one sample is analyzed, automated, over night runs often are performed for better laboratory efficiency. Typically, 24 hours stability is desired for all solutions and reagents that need to be prepared for each analysis. Mobile phases should be chosen to avoid stability problems, especially the use of amine additives or specific solvents. For example, mobile phase containing THF (tetra hydrofuran) are known to be susceptible to oxidation, therefore, the mobile phase should be prepared daily with fresh THF. Some buffered mobile phases cause problems for example, phosphate and acetate provide good media for microbial growth. Sodium oxide (0.1%) is often added to the mobile phase buffer to inhibit such growth, adding more than 5% of organic solvent is also effective. Long term column stability is critical for method ruggedness. Even the best HPLC column will eventually degrade and lose its initial performance, often as a function of the number of samples injected. System suitability System suitability experiments can be defined as tests to ensure that the method can generate results of acceptable accuracy and precision. The requirements for system suitability are usually developed after method development and validation have been completed. The criteria selected will be based on the actual performance of the method as determined during its validation. For example, if sample retention times forms part of the system suitability criteria, their variation (SD) during validation can be determined, system suitability might then require that retention times fall within a  ±3 SD range during routine performance of the method. The USP (2000) defines parameters that can be used to determine system suitability prior to analysis. These parameters include plate number (N), tailing factor, k and / or a, resolution (Rs) and relative standard deviation (RSD) of peak height or peak area for respective injections. The RSD of peak height or area of five injections of standard solution is normally accepted as one of the standard criteria. For an assay method of a major component, the RSD should typically be less than 1% for these five respective injections. The plate number and / or tailing factor are used if the run contains only one peak. For chromatographic separations with more than one peak, such as an internal standard assay or an impurity method, expected to contain many peaks, some measure of separations such as Rs is recommended. Reproducibility of tR or k value for a specific compound also defines system performance. The column performance can be defined in terms of column plate number ‘N’ is defined by N = 5.54 (tR / W ½)2 Where ‘tR’ is the retention time of the peak and ‘W ½Ã¢â‚¬â„¢ is the width of the peak at half peak height. The resolution of two adjacent peaks can be calculated by using the formula Rs = 1.18 (t2-t1) / W0.5.1 +W0.5.2 Where ‘t1’ and ‘t2’ are retention times of the adjacent peaks and W0.5.1 and W0.5.2 are the width of the peaks at half height. Rs = 2.0 or greater is a desirable target for method development. The retention factor k is given by the equation. k = (tR – t0) / t0 where ‘tR’ is the band retention time and t0 is the column dead time. The peak symmetry can be represented in terms of peak asymmetry factor and peak tailing factor, which can be calculated by using the following formula. Peak asymmetry factor = B /A Where ‘B’ is the distance at 50% peak height between leading edge to the perpendicular drawn from the peak maxima and ‘A’ is the width of the peak at half height. According to USP (2000) peak tailing factor can be calculated by using the formula T = W0.05 / 2f Where â€Å"W0.05† is the width of the peak at 5% height and â€Å"f† is the distance from the peak maximum to the leading edge of the peak, the distance being measured at a point 50% of the peak height from the base line.